SERVICES

Automation
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OpenTrons Robotic Liquid Handler

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Tecan Liquid Handling Robot

Hamilton Liquid Handling Robot

Tecan Liquid Handling Robot

BU Rate $55 per hour        External Rate $95 per hour

Our Tecan Freedom Evo 150 is configured with 8 individually addressable pipetting arms capable of pipetting volumes between 2-1000 uL each. The device is also configured with two heating/cooling blocks, as well as a robotic arm capable of moving plateware and consumables around its modular deck. This allows for the automation of several molecular biology processes including DNA assembly, transformation, PCR, NGS library prep, and more.

OpenTrons Robotic Liquid Handlers

BU Rate $30 per hour        External Rate $60 per hour

The OpenTrons Robotic Liquid Handler is equipped with two replaceable high-precision electronic pipettes that can be either single or multi-channels. There are 10 open deck slots available for various types of standard labwares, and the capabilities of the robotic liquid handler can be expanded with swappable on-deck modules.

Hamilton Liquid Handling Robot

BU Rate $55 per hour        External Rate $95 per hour

The Hamilton Liquid Handler achieves high accuracy, precision, and repeatability from sub-microliter to large volumes. This allows for the automation of several molecular biology processes including DNA assembly, transformation, PCR, NGS library prep, and more. Contact us and request for more information in case you are interested in using it.

High-throughput DNA Assembly and Cloning
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Scarless Assembly & Cloning

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Modular Assembly & Cloning

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Plasmid DNA Miniprep

Plasmid DNA Miniprep

BU Rate $4 per sample        External Rate $12 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Scarless Assembly & Cloning

BU Rate $160 to $520+ per sample (by pieces provided)        External Rate $200 to $650+ per sample (by pieces provided)

The DAMP Lab offers multiple fragment assembly and cloning using bacteria-based gene assembly systems with Gibson assembly methodology. All final DNA constructs are sequence verified.

Modular Assembly & Cloning

BU Rate $140 to $420+ per sample (by pieces provided)        External Rate $180 to $550+ per sample (by pieces provided)

The DAMP Lab offers multiple fragment assembly and cloning using bacteria-based gene assembly systems with Modular cloning methodology. All final DNA constructs are sequence verified.

Miniprep & Glycerol Stock

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Purify DNA From Reaction

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Purify DNA From Agarose Gel Extraction

Purify DNA From Agarose Gel Extraction

BU Rate $4 per sample        External Rate $18 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Plasmid DNA Miniprep & Glycerol Stock

BU Rate $6 per sample        External Rate $15 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Purify DNA From Reaction

BU Rate $4 per sample        External Rate $12 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Clean Up & Concentrate DNA

RNA Extraction

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mRNA Enrichment

mRNA Enrichment

BU Rate $38 per sample        External Rate $58 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Clean Up & Concentrate DNA

BU Rate $4 per sample        External Rate $25 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

RNA Extraction

BU Rate $35 per sample        External Rate $55 per sample

The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.

Fluorescence-based Assays
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96-well Plate Format E.coli Growth

E. coli Growth Fluorescence Microscope Assay

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Flow Cytometry

Flow Cytometry

BU Rate to be determined

Due to their various ranges of sensitivity, types of fluorophores, readout modes, and ease of operation, fluorescence-based assays are widely used in high-throughput screening in a broad range of activities in life-science research, e.g. such as molecular dynamics and interactions, enzymatic activities, signal transduction, cell viability, and quantification of molecules, organelles, or cells.

96-Well Plate Format E. coli Growth

BU Rate $5 per sample        External Rate $20 per sample

The functionality of genetic fluorescence-based systems can be measured by a multi-mode 96-well microplate reader under various growth/incubation optimal/non-optimal conditions. Both average fluorescence intensities from various modes and average cell biomass (measured by optical densities) of each individual sample are monitored from cells at certain sampling intervals and periods.

E. coli Growth Fluorescence Microscope Assay

BU Rate $5 per sample        External Rate $20 per sample

Single-cell data can be visualized by a set of epi-fluorescence light source, and a filter cube containing an excitation filter, a dichroic mirror, and an emission filter of a specific fluorescence mode using an inverted microscope. Along with fluorescence images, phase contrast images of the same location can also be captured by a CCD camera for concomitant cell morphological analysis. Microscopy images are captured using MicroManager, open-source software tool, and analyzed using customized scripts in MATLAB.

Materials Repository and Storage
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Glycerol Stocks of E. coli Cells

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Plasmid, DNA Fragments, Oligos Storage

Bacteria Storage LB Agar Plate 

Bacteria Storage LB Agar Plate

BU Rate $3 per sample        External Rate $10 per sample

LB agar plates are frequently used to isolate individual colonies of bacteria carrying a specific plasmid. DAMP lab offers up to 1 month of storage time for bacteria LB agar plates.

Glycerol Stocks of E. coli Cells

BU Rate $2 per sample        External Rate $8 per sample

The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive for long term storage. DAMP lab offers up to 2 years of storage time for glycerol stocks of Bacteria cell culture.

Plasmid, DNA Fragments, Oligos Storage

BU Rate $2 per sample        External Rate $8 per sample

DAMP lab offers up to 2 years of storage time for plasmid, DNA fragments, and oligos storage.

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Liquid Culture Overnight

Liquid Culture Overnight

BU Rate $3 per sample        External Rate $10 per sample

A liquid bacteria culture is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid DNA for experimental use. DAMP lab offers up to 2 days of storage time for the overnight inoculums of bacteria cell cultures.

Transcriptomics
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cDNA Library Prep

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Bioanalyzer

RNA Sequencing

RNA Sequencing

Rate $1880 per 150 cycles (midi), $2130 per 75 cycles (high)

The DAMP Lab currently provides mRNA enrichment for both bacterial RNA (rRNA removal) and mammalian RNA (poly-A selection) inputs, cDNA library preparation using the NEB Ultra kit, indexing and quality control before performing high-throughput sequencing via Illumina HiSeq 2000.

cDNA Library Prep

BU Rate $70 per sample        External Rate $120 per sample

The DAMP Lab offers transcriptomics services tailoring to the research objectives of our clients. We are able to advise you on various stages of experimental and analytical work, from project design, material collection, to downstream bioinformatics analysis.

Bioanalyzer

BU Rate $120 per DNA-RNA chip pair        External Rate $160 per DNA-RNA chip pair

The DAMP Lab offers transcriptomics services tailoring to the research objectives of our clients. We are able to advise you on various stages of experimental and analytical work, from project design, material collection, to downstream bioinformatics analysis.

 
 
 
 

CONTACT US

DAMP Lab

610 Commonwealth Ave, 4th Floor
Boston, MA 02215

E-mail: damplab@bu.edu
Tel: (617) 358-6238

For inquiries or more information, please drop us a message. We'd love to connect with you!

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