High-throughput DNA Assembly and Cloning
High-throughput DNA Assembly and Cloning
SERVICES
Automation
OpenTrons Robotic Liquid Handler
Hamilton Liquid Handling Robot
OpenTrons Robotic Liquid Handlers
BU Rate $250* External Rate $375*
The OpenTrons Robotic Liquid Handler is equipped with two replaceable high-precision electronic pipettes that can be either single or multi-channels. There are 10 open deck slots available for various types of standard labwares, and the capabilities of the robotic liquid handler can be expanded with swappable on-deck modules.
*per 5 hour consulting project
Hamilton Liquid Handling Robot
BU Rate $500* External Rate $750*
The Hamilton Liquid Handler achieves high accuracy, precision, and repeatability from sub-microliter to large volumes. This allows for the automation of several molecular biology processes including DNA assembly, transformation, PCR, NGS library prep, and more. Contact us and request for more information in case you are interested in using it.
*per 5 hour consulting project
DNA Assembly and Cloning
Gibson Assembly
Modular Cloning (Golden Gate)
Plasmid DNA Miniprep
Plasmid DNA Miniprep
BU Rate $4 per sample External Rate $12 per sample
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Scarless Assembly & Cloning
BU Rate $160 to $520+ per sample (by pieces provided) External Rate $200 to $650+ per sample (by pieces provided)
The DAMP Lab offers multiple fragment assembly and cloning using bacteria-based gene assembly systems with Gibson assembly methodology. All final DNA constructs are sequence verified.
Modular Assembly & Cloning
The DAMP Lab offers multiple fragment assembly and cloning using bacteria-based gene assembly systems with Modular cloning methodology. All final DNA constructs are sequence verified.
Miniprep & Glycerol Stock
Purify DNA From Reaction
Purify DNA From Agarose Gel Extraction
Purify DNA From Agarose Gel Extraction
BU Rate $4 per sample External Rate $18 per sample
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Plasmid DNA Miniprep & Glycerol Stock
BU Rate $6 per sample External Rate $15 per sample
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Purify DNA From Reaction
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Clean Up & Concentrate DNA
RNA Extraction
mRNA Enrichment
mRNA Enrichment
BU Rate $38 per sample External Rate $58 per sample
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Clean Up & Concentrate DNA
BU Rate $4 per sample External Rate $25 per sample
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
RNA Extraction
The DAMP Lab provides high quality, nucleic acid isolation from client samples of various sample types including bacteria, genomic DNA isolation, as well as DNA and RNA isolation from mammalian cells using commercially available nucleic acid purification kits. We accept samples in various formats: agar plates, stripes, glycerol stocks, and liquid cultures.
Fluorescence-based Assays
96-well Plate Format E.coli Growth
E. coli Growth Fluorescence Microscope Assay
Flow Cytometry
Flow Cytometry
BU Rate to be determined
Due to their various ranges of sensitivity, types of fluorophores, readout modes, and ease of operation, fluorescence-based assays are widely used in high-throughput screening in a broad range of activities in life-science research, e.g. such as molecular dynamics and interactions, enzymatic activities, signal transduction, cell viability, and quantification of molecules, organelles, or cells.
96-Well Plate Format E. coli Growth
BU Rate $5 per sample External Rate $20 per sample
The functionality of genetic fluorescence-based systems can be measured by a multi-mode 96-well microplate reader under various growth/incubation optimal/non-optimal conditions. Both average fluorescence intensities from various modes and average cell biomass (measured by optical densities) of each individual sample are monitored from cells at certain sampling intervals and periods.
E. coli Growth Fluorescence Microscope Assay
Single-cell data can be visualized by a set of epi-fluorescence light source, and a filter cube containing an excitation filter, a dichroic mirror, and an emission filter of a specific fluorescence mode using an inverted microscope. Along with fluorescence images, phase contrast images of the same location can also be captured by a CCD camera for concomitant cell morphological analysis. Microscopy images are captured using MicroManager, open-source software tool, and analyzed using customized scripts in MATLAB.
Materials Repository and Storage
Glycerol Stocks of E. coli Cells
Plasmid, DNA Fragments, Oligos Storage
Bacteria Storage LB Agar Plate
Bacteria Storage LB Agar Plate
BU Rate $3 per sample External Rate $10 per sample
LB agar plates are frequently used to isolate individual colonies of bacteria carrying a specific plasmid. DAMP lab offers up to 1 month of storage time for bacteria LB agar plates.
Glycerol Stocks of E. coli Cells
BU Rate $2 per sample External Rate $8 per sample
The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive for long term storage. DAMP lab offers up to 2 years of storage time for glycerol stocks of Bacteria cell culture.
Plasmid, DNA Fragments, Oligos Storage
DAMP lab offers up to 2 years of storage time for plasmid, DNA fragments, and oligos storage.
Liquid Culture Overnight
Liquid Culture Overnight
A liquid bacteria culture is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid DNA for experimental use. DAMP lab offers up to 2 days of storage time for the overnight inoculums of bacteria cell cultures.
Transcriptomics
cDNA Library Prep
Bioanalyzer
RNA Sequencing
Bioanalyzer
BU Rate $120 per DNA-RNA chip pair External Rate $160 per DNA-RNA chip pair
The DAMP Lab offers transcriptomics services tailoring to the research objectives of our clients. We are able to advise you on various stages of experimental and analytical work, from project design, material collection, to downstream bioinformatics analysis.
RNA Sequencing
Rate $1880 per 150 cycles (midi), $2130 per 75 cycles (high)
The DAMP Lab currently provides mRNA enrichment for both bacterial RNA (rRNA removal) and mammalian RNA (poly-A selection) inputs, cDNA library preparation using the NEB Ultra kit, indexing and quality control before performing high-throughput sequencing via Illumina HiSeq 2000.
cDNA Library Prep
The DAMP Lab offers transcriptomics services tailoring to the research objectives of our clients. We are able to advise you on various stages of experimental and analytical work, from project design, material collection, to downstream bioinformatics analysis.