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High-throughput DNA Assembly and Cloning
High-throughput DNA Assembly and Cloning
Service Request Form
DNA Assembl and Cloning
Gibson Assembly
Modular Cloning (Golden Gate)
Modular Gibson Assembly
Restriction Digestion
Restriction Ligation
Mutagenesis
Mutagenesis by Inverse PCR
PCR Reaction
Perform PCR Reaction
Colony PCR
Temperature Gradient Test
Fluorescence-base Assays
In vivo 96-well Plate Format E. Coli Growth w/ Fluoresence and Biomass Assay
Flow Cytometry
Perform qPCR Assay
Culturing and Media
Cell Culture Induction and Selection
Cell Lysate Production
Protein Production and Purification from Cell Lysate
Cell Transformation
Prepare Liquid Culture Overnight Inoculum
E. coli LB Growth Media
E. coli M9 Growth Media
E. Coli X Agar Plate Media + Y Antibiotics
DNA and RNA
Purify DNA from Reaction
Perform Gel Electrophoresis Analysis
Purify DNA from Agarose Gel Extraction
Clean Up and Concentrate DNA
DNA/RNA Ethanol Precipitation
RNA Extraction
Plasmid DNA Miniprep
Plasmid DNA Miniprep and Glycerol Stock
Plasmid DNA Midiprep
Plasmid DNA Maxiprep
Send Sample to Sequencing
Rehydrate and Suspend DNA Oligo in Solution
Ordering DNA Fragments from Third-party
Transciptomics (NGS)
mRNA enrichment
cDNA Library Preparation
DNA and RNA Quality Analysis with Bioanalyzer
RNA Sequencing (NGS) from Third-party
Materials Repository and Storage
Plasmid
Glycerol Stocks of Bacteria Cell Culture
Overnight Inoculums of Bacteria Cell Culture
Storage of Bacteria on LB Agar Plate with Appropriate Resistance
Materials Repository and Storage
Plasmid
Glycerol Stocks of Bacteria Cell Culture
Overnight Inoculums of Bacteria Cell Culture
Storage of Bacteria on LB Agar Plate with Appropriate Resistance
Bundles
Bundle1: Scarless Assembly and Cloning Fragments Provided: >2 pieces
Bundle2: Scarless Assembly and Cloning: >2 pieces + Ordering
Bundle3: Modular Assembly and Cloning Fragments Provided: >2 pieces
Bundle4: Modular Assembly and Cloning: >2 pieces + Ordering
Bundle5: BioBrick Assembly and Cloning Fragments Provided: >2 pieces
Bundle6: BioBrick Assembly and Cloning: >2 pieces + Ordering
Bundle7: RNA-Sequencing (NGS)
Bundle8: COVID-19 Testing
Bundle9: Protein Production
Bundle10: MonkeyPox Testing (coming soon)
Bundle11: Influenza B Testing (coming soon)
Automation
OpenTrons Robotic Liquid Handler Assisted (OT-2)
Hamilton Liquid Handling Robot Assist (Microlab STAR)
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